This document specifies the protocol for the verification of reference methods and validated alternative methods for implementation in the user laboratory. This document is applicable to the verification of methods used for the analysis (detection and/or quantification), confirmation and typing of microorganisms in: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production, handling; — samples from the prima...view more

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    • Standard
      80 pages
      French language
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    • Standard
      70 pages
      English language
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    • Draft
      69 pages
      English language
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    • Draft
      79 pages
      French language

This document specifies a method for the direct enumeration of potentially enteropathogenic V. parahaemolyticus (tdh and/or trh positive) and/or the enumeration of total V. parahaemolyticus in seafood.

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    • Technical specification
      27 pages
      English language
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    • Draft
      27 pages
      English language

This document specifies the general principles and the technical protocols for single-laboratory validation of methods for microbiology in the food chain. The protocols in this document only validate the method for the laboratory conducting the study. This document is applicable to single-laboratory validation of: — methods used in the analysis (detection or quantification) of microorganisms in: — products intended for human consumption; — products intended for animal feeding; — environmental sa...view more

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    • Standard
      46 pages
      English language
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    • Standard
      49 pages
      French language
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    • Standard
      49 pages
      French language
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    • Standard
      46 pages
      English language

This document specifies the general principles and the technical protocols (based on orthogonal, factorial studies) for the validation of non-proprietary methods for microbiology of the food chain. This document is applicable to the validation of methods used for the analysis (detection or quantification) of microorganisms in: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production, handling; — samples fro...view more

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    • Standard
      38 pages
      French language
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    • Standard
      34 pages
      English language
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    • Standard
      38 pages
      French language
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    • Standard
      34 pages
      English language
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    • Standard
      14 pages
      French language
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    • Standard
      13 pages
      English language
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    • Standard
      14 pages
      French language
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    • Standard
      13 pages
      English language

This document specifies rules for the preparation of samples of milk and milk products and their suspensions for microbiological examination when the samples require a different preparation from the general methods specified in ISO 6887-1. This document excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards. This document is intended to be used in conjunction with ISO 6887-1. This documen...view more

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    • Standard
      11 pages
      French language
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    • Standard
      11 pages
      English language
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    • Standard
      27 pages
      French language
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    • Standard
      27 pages
      English language
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    • Standard
      27 pages
      French language
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    • Standard
      27 pages
      English language
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    • Standard
      1 page
      English language
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    • Standard
      1 page
      French language

This document specifies the general principle and the technical protocol for the validation of alternative confirmation methods for microbiology in the food chain. This document compares the result of the alternative confirmation method against the confirmation procedure of a reference method or, if needed, a reference confirmation method (e.g. whole genome sequencing). This document is applicable to the validation of alternative confirmation methods used for the analysis (detection or quantific...view more

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    • Standard
      26 pages
      French language
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    • Standard
      25 pages
      English language

This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain. It is applicable to the quantitative analysis of: — products intended for human consumption or the feeding of animals; — environmental samples in the area of food production and food handling; — samples at the stage of primary production. The quantitative analysis is typically carried out by enumeration o...view more

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    • Standard
      39 pages
      French language
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    • Standard
      38 pages
      English language

This document specifies a horizontal method for the enumeration of psychrotrophic microorganisms that are able to grow and form colonies on a solid agar culture medium after aerobic incubation at 6,5 °C. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage. NOTE Annex B specifies a rapid method for the estimated en...view more

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    • Standard
      10 pages
      French language
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    • Standard
      10 pages
      English language

This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or ot...view more

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    • Standard
      40 pages
      English language
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    • Standard
      47 pages
      French language

This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products. The use of this document can be extended to yeasts that do not form mycelium.

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    • Standard
      29 pages
      French language
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    • Standard
      28 pages
      English language

This document specifies requirements and gives guidelines for the organization of proficiency testing (PT) schemes for microbiological examinations of a) foods and beverages, b) feeding animals, c) environmental samples from food and feed production and handling, and d) primary production stages. This document is also applicable to the microbiological examination of water where water is either used in food production or is regarded as a food in national legislation. This document relates to the ...view more

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    • Standard
      36 pages
      French language
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    • Standard
      33 pages
      English language
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    • Standard
      4 pages
      French language
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    • Standard
      5 pages
      English language

This document specifies horizontal methods for sampling techniques using contact plates, stick swabs, sponges and cloths on surfaces in the food chain environment in order to detect and enumerate culturable microorganisms such as pathogenic or non-pathogenic bacteria or yeasts and moulds. NOTE The term "environment" means any item in contact with the food product or likely to represent a contamination or recontamination source; for example, material, premises or operators. This document does not...view more

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    • Standard
      11 pages
      French language
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    • Standard
      11 pages
      English language

ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to - products intended for human consumption, - products intended for feeding animals, - environmental samples in the area of food production and food hand...view more

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    • Standard
      11 pages
      French language
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    • Standard
      11 pages
      English language
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    • Standard
      7 pages
      French language
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    • Standard
      7 pages
      English language

ISO 13722:2017 specifies a method for the enumeration of viable Brochothrix spp. by means of a colony-count technique. ISO 13722:2017 is especially applicable to meat and meat products, but is also suitable for the examination of the following: - other products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage.

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    • Standard
      9 pages
      English language

ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. ISO 21872-1:2017 is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling.

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    • Standard
      37 pages
      French language
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    • Standard
      33 pages
      English language

ISO 10272-2:2017 specifies a horizontal method for the enumeration of Campylobacter spp. It is applicable to - products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage such as animal faeces, dust, and swabs.

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    • Standard
      19 pages
      English language
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    • Standard
      19 pages
      French language

ISO 10272-1:2017 specifies a horizontal method for the detection by enrichment or direct plating of Campylobacter spp. It is applicable to - products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage such as animal faeces, dust, and swabs.

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    • Standard
      24 pages
      English language
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    • Standard
      24 pages
      French language

ISO 19343:2017 specifies a high performance liquid chromatography (HPLC) method to analyse histamine in fish and fishery products (fish sauces, fish maturated by enzyme in brine, etc.) intended for human consumption.

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    • Standard
      14 pages
      French language
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    • Standard
      14 pages
      English language

ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of primary production, food production and food handling. This method is applicable - when the microorganisms sought are expected to need resuscitation by enrichment, and - when the number sought is expected to be below 100 per millilitre or per gram of test sample. A limita...view more

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    • Standard
      19 pages
      French language
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    • Standard
      17 pages
      English language

ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of primary production, food production and food handling. This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample. The most probable number (MPN) technique, as included in ISO 21528‑1, is generall...view more

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    • Standard
      15 pages
      French language
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    • Standard
      15 pages
      English language

ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits. ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption. Other staphylococcal enterotoxins such as types S...view more

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    • Standard
      21 pages
      French language
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    • Standard
      22 pages
      English language

ISO 11290-2:2017 specifies a horizontal method for - the enumeration of L. monocytogenes, and - the enumeration of Listeria spp. (including L. monocytogenes). ISO 11290-2:2017 is applicable to - products intended for human consumption and for the feeding of animals, and - environmental samples in the area of food production and food handling.

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    • Standard
      29 pages
      French language
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    • Standard
      29 pages
      English language

ISO 11290-1:2017 specifies a horizontal method for - the detection of L. monocytogenes, and - the detection of Listeria spp. (including L. monocytogenes). ISO 11290-1:2017 is applicable to - products intended for human consumption and for the feeding of animals, and - environmental samples in the area of food production and food handling.

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    • Standard
      36 pages
      English language
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    • Standard
      37 pages
      French language

ISO 22964:2017 specifies a horizontal method for the detection of Cronobacter spp. Subject to the limitations discussed in the introduction, this document is applicable to - food products and ingredients intended for human consumption and the feeding of animals, and - environmental samples in the area of food production and food handling.

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    • Standard
      23 pages
      English language
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    • Standard
      23 pages
      French language
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    • Standard
      5 pages
      French language
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    • Standard
      4 pages
      English language

ISO 10273:2017 specifies a horizontal method for the detection of Y. enterocolitica associated with human disease. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of food production and food handling.

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    • Standard
      41 pages
      French language
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    • Standard
      40 pages
      English language

ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption. ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result. ISO 6...view more

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    • Standard
      28 pages
      French language
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    • Standard
      26 pages
      English language

ISO 6887-3:2017 specifies rules for the preparation of fish and fishery product samples and their suspension for microbiological examination when the samples require a different preparation from the methods described in ISO 6887‑1. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination. ISO 6887-3:2017 includes special procedures for sampling raw molluscs, tunicates and echinoderms from primary production areas. NOTE 1 Sam...view more

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    • Standard
      17 pages
      French language
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    • Standard
      16 pages
      English language

ISO 6887-4:2017 specifies rules for the preparation of samples and dilutions for the microbiological examination of specific food products not covered in other parts of ISO 6887, which deal with more general categories. This document covers a wide range of miscellaneous products, but does not include new products brought on to the market after publication. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination. ISO 6887-4:...view more

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    • Standard
      17 pages
      French language
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    • Standard
      16 pages
      English language

ISO 6887-2:2017 specifies rules for the preparation of meat and meat product samples and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887‑1. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination. ISO 6887-2:2017 excludes preparation of samples for both enumeration and detection test methods where preparation details are specified in the ...view more

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    • Standard
      10 pages
      French language
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    • Standard
      9 pages
      English language

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated f...view more

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    • Standard
      49 pages
      French language
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    • Standard
      48 pages
      English language

ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling; - samples from the primary production stage such as animal faeces, dust, and swabs. With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture step...view more

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    • Standard
      50 pages
      English language
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    • Standard
      50 pages
      French language

ISO 18465:2017 describes the quantitative analysis of the emetic toxin cereulide using high performance liquid chromatography (HPLC) or ultra performance liquid chromatography (UHPLC) connected to a tandem mass spectrometer (LC-MS/MS). ISO 18465:2017 is applicable to the analysis of the toxin in products intended for human consumption.

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    • Standard
      14 pages
      English language
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    • Standard
      15 pages
      French language

ISO 16140-1:2016 defines general terms and definitions relating to method validation of microbiology in the food chain. It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in - products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage.

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    • Standard
      12 pages
      English language
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    • Standard
      13 pages
      French language

ISO 16140-2:2016 specifies the general principle and the technical protocol for the validation of alternative, mostly proprietary, methods for microbiology in the food chain. Validation studies according to ISO 16140-2:2016 are intended to be performed by organizations involved in method validation. It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in - products intended for human consumption, - products intended for animal feeding, - ...view more

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    • Standard
      69 pages
      French language
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    • Standard
      66 pages
      English language

ISO 17468:2016 gives technical requirements and guidance on the establishment or revision of standardized reference methods for the analysis (detection or quantification) of microorganisms in - products intended for human consumption and for the feeding of animals, - environmental samples in the area of food/feed production and food/feed handling, and - samples from the primary production stage. It defines the technical stage (or early stage) of the establishment of a new standardized reference ...view more

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    • Standard
      6 pages
      French language
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    • Standard
      5 pages
      English language

ISO 18744:2016 specifies a method that is applicable for the detection and enumeration of Cryptosporidium oocysts and Giardia cysts on or in food products that are described herein as fresh leafy green vegetables and berry fruits. With suitable controls, it may also be applicable for the examination of other fresh produce. The microscopy descriptions are for Cryptosporidium spp. oocysts and Giardia duodenalis cysts of size ranges which include those species (Cryptosporidium) or assemblages (Giar...view more

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    • Standard
      22 pages
      French language
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    • Standard
      21 pages
      English language
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    • Standard
      22 pages
      French language
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    • Standard
      21 pages
      English language

ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosi...view more

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    • Technical specification
      30 pages
      French language
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    • Technical specification
      30 pages
      English language

ISO 18743:2015 specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella. This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods. The method described i...view more

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    • Standard
      16 pages
      French language
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    • Standard
      15 pages
      English language

ISO 17604:2015 specifies sampling methods for the detection and enumeration of microorganisms on the surface of carcasses or parts of carcasses of slaughtered meat animals. The microbiological sampling can be carried out as part of - process hygiene control (to validate and or verify process control, e.g. total counts and Enterobacteriaceae) in slaughter establishments for large mammals, poultry, and game, - risk-based assurance systems for product safety, and - monitoring or surveillance progra...view more

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    • Standard
      14 pages
      French language
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    • Standard
      13 pages
      English language

ISO/TS 17728:2015 applies to the collection of samples before submission to the laboratory for microbiological examination. It provides general instructions and specific requirements for obtaining samples and for transport to the laboratory. Sampling plans are not included in the scope of this Technical Specification.

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    • Technical specification
      18 pages
      French language
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    • Technical specification
      17 pages
      English language

ISO 16649-3:2015 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling. The method is suitable...view more

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    • Standard
      10 pages
      French language
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    • Standard
      10 pages
      English language
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    • Standard
      10 pages
      French language
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    • Standard
      10 pages
      English language

ISO/TR 6579-3:2014 gives guidance on the procedure for serotyping Salmonella serovars and is applicable to the serotyping of pure cultures of Salmonella spp., independent of the source from which they are isolated.

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    • Technical report
      35 pages
      French language
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    • Technical report
      33 pages
      English language

ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses. ISO 11133:2014...view more

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    • Standard
      103 pages
      French language
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    • Standard
      99 pages
      English language
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    • Standard
      103 pages
      French language
    • sale 15% off
    • Standard
      99 pages
      English language